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New England Biolabs
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ELISA SYSTEMS Pty Ltd
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Corning Life Sciences
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Corning Life Sciences
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Clinical and Laboratory Standards Institute
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Clinical and Laboratory Standards Institute
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Corning Life Sciences
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Becton Dickinson
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Avantor
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Image Search Results
Journal: bioRxiv
Article Title: EZ-SPOTs: A simple and robust high-throughput liquid handling platform
doi: 10.1101/2024.05.13.594031
Figure Lengend Snippet: Experimental design using the EZ-SPOTs platform for ASTs. A) EZ-SPOTs plates are interfaced with 96-well microtiter plates by lining up and flipping the EZ-SPOTs plate on top of the 96-well microtiter plate, clamping two sides, flipping the two plates upside down, and shaking until well-mixed; B) Schematics of the EZ-SPOTs antibiotic plate design which contains three replicates of six antibiotics at five different volumes and six coated areas for controls and the EZ-SPOTs cell plate which is loaded with one isolate and has spaces for three positive controls and three negative controls; C) a 96-well microtiter plate was filled with media, interfaced with the EZ-SPOTs antibiotic plate which was loaded with a six-slot loader, then the resulting 96-well plate was interfaced with the EZ-SPOTs cell plate which was loaded with a single-slot loader and each 96-well microtiter plate was incubated until an OD600 reading. The EZ-SPOTs antibiotic plate was rinsed and reused for each isolate and the EZ-SPOTs cell plate was bleached for two minutes, rinsed, and reused for each isolate.
Article Snippet: EZ-SPOTs plates were interfaced with filled 96-well
Techniques: Incubation